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Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome
JoVE Journal
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JoVE Journal Biyokimya
Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

DOI:
10.3791/63552-v

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06:48 min

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March 23, 2022

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, , ,
1Medical School,Swansea University, 2Department of Basic Medical Sciences, Neuroscience and Sense Organs,University of Bari Aldo Moro

Bölümler

  • 00:04Introduction
  • 00:53Isolation of Leukocytes by Dextran Sedimentation of Red Blood Cells
  • 02:57“Mini-Extraction” of Lipids from Isolated Leukocytes
  • 04:03Lipid Analysis by MALDI-TOF/MS
  • 05:00Results: Diagnosis of Barth Syndrome Using Leukocyte CL Fingerprints and (MLCL + CLi)/CLm Ratios
  • 06:06Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol shows how to obtain a mass spectrometric "fingerprint" of leukocyte cardiolipin for the diagnosis of Barth syndrome. The assessment of elevated monolysocardiolipin to cardiolipin ratio discriminates patients with Barth syndrome from control heart failure patients with 100% sensitivity and specificity.

Etiketler

Cardiolipin FingerprintingLeukocytesMass SpectrometryBarth SyndromeMonolysocardiolipinMALDI-TOFBlood Sample ProcessingOsmotic ShockLipid Extraction

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