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Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome
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Biochimie
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Journal JoVE Biochimie
Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

2,196 Views

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06:48 min

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March 23, 2022

DOI:

10.3791/63552-v

DOI:
10.3791/63552-v

•

06:48 min
March 23, 2022

•

2 Views
, , ,
1Medical School,Swansea University, 2Department of Basic Medical Sciences, Neuroscience and Sense Organs,University of Bari Aldo Moro

Summary

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This protocol shows how to obtain a mass spectrometric "fingerprint" of leukocyte cardiolipin for the diagnosis of Barth syndrome. The assessment of elevated monolysocardiolipin to cardiolipin ratio discriminates patients with Barth syndrome from control heart failure patients with 100% sensitivity and specificity.

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Cardiolipin FingerprintingLeukocytesMass SpectrometryBarth SyndromeMonolysocardiolipinMALDI-TOFBlood Sample ProcessingOsmotic ShockLipid Extraction

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Cite this Article

Angelini, R., Russo, S., Corcelli, A., Lobasso, S. Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome. J. Vis. Exp. (181), e63552, doi:10.3791/63552 (2022).

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