Journal
/
生物化学
/
Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

DOI:
10.3791/63552-v

•

06:48 min

•

March 23, 2022

•

, , ,
1Medical School,Swansea University, 2Department of Basic Medical Sciences, Neuroscience and Sense Organs,University of Bari Aldo Moro

Chapters

  • 00:04Introduction
  • 00:53Isolation of Leukocytes by Dextran Sedimentation of Red Blood Cells
  • 02:57“Mini-Extraction” of Lipids from Isolated Leukocytes
  • 04:03Lipid Analysis by MALDI-TOF/MS
  • 05:00Results: Diagnosis of Barth Syndrome Using Leukocyte CL Fingerprints and (MLCL + CLi)/CLm Ratios
  • 06:06Conclusion

Summary

自动翻译

自动翻译

This protocol shows how to obtain a mass spectrometric "fingerprint" of leukocyte cardiolipin for the diagnosis of Barth syndrome. The assessment of elevated monolysocardiolipin to cardiolipin ratio discriminates patients with Barth syndrome from control heart failure patients with 100% sensitivity and specificity.

Tags

Keywords: Cardiolipin FingerprintingLeukocytesMass SpectrometryBarth SyndromeMonolysocardiolipinMALDI-TOFBlood Sample ProcessingOsmotic ShockLipid Extraction

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/63552?article_type=v

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
check_url/cn/63552?article_type=v

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
check_url/cn/63552?article_type=v

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
check_url/cn/63552?article_type=v

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
check_url/cn/63552?article_type=v

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
check_url/cn/63552?article_type=v

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
check_url/cn/63552?article_type=v

Nitropeptide Profiling and Identification Illustrated by Angiotensin II
check_url/cn/63552?article_type=v

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
check_url/cn/63552?article_type=v

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
check_url/cn/63552?article_type=v

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Read Article