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次世代シーケンシングを使用して、CD4プロモーター近傍のCAS9誘導二本鎖切断の修復に関連する変異を同定
JoVE Journal
Genetics
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JoVE Journal Genetics
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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次世代シーケンシングを使用して、CD4プロモーター近傍のCAS9誘導二本鎖切断の修復に関連する変異を同定

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2,246 Views

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06:59 min

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March 31, 2022

DOI:

10.3791/62583-v

DOI:
10.3791/62583-v

•

06:59 min
March 31, 2022

•

3 Views
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1Division of Biology,Kansas State University, 2Kansas State Veterinary Diagnostic Laboratory,Kansas State University, 3Department of Diagnostic Medicine/Pathobiology,Kansas State University

Summary

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ここでは、CD4プロモーター近傍の二本鎖切断修復に関連する変異を同定するために使用できるsgRNA/CAS9エンドヌクレアーゼおよび次世代シーケンシングプロトコルを紹介します。

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Cite this Article

Hu, C., Doerksen, T., Bugbee, T., Wallace, N. A., Palinski, R. Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter. J. Vis. Exp. (181), e62583, doi:10.3791/62583 (2022).

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