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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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March 31, 2022
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Presented here is sgRNA/CAS9 endonuclease and next-generation sequencing protocol that can be used to identify the mutations associated with double strand break repair near the CD4 promoter.
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Hu, C., Doerksen, T., Bugbee, T., Wallace, N. A., Palinski, R. Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter. J. Vis. Exp. (181), e62583, doi:10.3791/62583 (2022).
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