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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Genetik
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JoVE Journal Genetik
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

DOI:
10.3791/62583-v

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06:59 min

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March 31, 2022

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, , , ,
1Division of Biology,Kansas State University, 2Kansas State Veterinary Diagnostic Laboratory,Kansas State University, 3Department of Diagnostic Medicine/Pathobiology,Kansas State University

Bölümler

  • 00:05Introduction
  • 00:41Cell Plating
  • 02:21Transfection
  • 04:42Results: Identifying the Mutations Associated with Double Strand Break Repair near the CD4 Promoter
  • 06:15Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Presented here is sgRNA/CAS9 endonuclease and next-generation sequencing protocol that can be used to identify the mutations associated with double strand break repair near the CD4 promoter.

Etiketler

Next Generation SequencingCAS9Double-strand BreakCD4 PromoterMutation IdentificationHuman KeratinocytesTransfectionCRISPRGene Editing

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