Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Changkun Hu; Tyler Doerksen; Taylor Bugbee; Nicholas A. Wallace; Rachel Palinski

doi:10.3791/62583

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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

JoVE Journal
Genetica

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JoVE Journal Genetica

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

2,246 Views

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06:59 min

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March 31, 2022

DOI:

10.3791/62583-v

DOI:

10.3791/62583-v

•

06:59 min

March 31, 2022

•

3 Views

Changkun Hu*¹, Tyler Doerksen*², Taylor Bugbee¹, Nicholas A. Wallace¹, Rachel Palinski^2,3

¹Division of Biology,Kansas State University, ²Kansas State Veterinary Diagnostic Laboratory,Kansas State University, ³Department of Diagnostic Medicine/Pathobiology,Kansas State University

Summary

Presented here is sgRNA/CAS9 endonuclease and next-generation sequencing protocol that can be used to identify the mutations associated with double strand break repair near the CD4 promoter.

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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Summary

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