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Next Generation Sequencing gebruiken om mutaties te identificeren die verband houden met reparatie van een cas9-geïnduceerde dubbelstrengsbreuk in de buurt van de CD4-promotor
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Genetics
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JoVE Journal Genetics
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Next Generation Sequencing gebruiken om mutaties te identificeren die verband houden met reparatie van een cas9-geïnduceerde dubbelstrengsbreuk in de buurt van de CD4-promotor

Nederlands

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2,246 Views

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06:59 min

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March 31, 2022

DOI:

10.3791/62583-v

DOI:
10.3791/62583-v

•

06:59 min
March 31, 2022

•

3 Views
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1Division of Biology,Kansas State University, 2Kansas State Veterinary Diagnostic Laboratory,Kansas State University, 3Department of Diagnostic Medicine/Pathobiology,Kansas State University

Summary

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Hier gepresenteerd is sgRNA / CAS9 endonuclease en next-generation sequencing protocol dat kan worden gebruikt om de mutaties te identificeren die geassocieerd zijn met dubbelstrengs breukherstel in de buurt van de CD4-promotor.

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Cite this Article

Hu, C., Doerksen, T., Bugbee, T., Wallace, N. A., Palinski, R. Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter. J. Vis. Exp. (181), e62583, doi:10.3791/62583 (2022).

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