Lin, C., Zhu, D., Markby, G., Corcoran, B. M., Farquharson, C., Macrae, V. E. Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification. J. Vis. Exp. (129), e56126, doi:10.3791/56126 (2017).