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Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
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JoVE Journal Developmental Biology
Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

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5,882 Views

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12:59 min

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February 15, 2020

DOI:

10.3791/59924-v

DOI:
10.3791/59924-v

•

12:59 min
February 15, 2020

•

4 Views
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1Stem Cells and Regenerative Medicine Centre,Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangaluru-575018, Karnataka, India

Summary

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This protocol demonstrates the simultaneous detection of reactive oxygen species (ROS), live cells, and dead cells in live primary cultures from mouse ocular surface cells. 2',7'-Dichlorofluoresceindiacetate, propidium iodide, and Hoechst staining are used to assess the ROS, dead cells, and live cells, respectively, followed by imaging and analysis.

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Oxidative DamagePrimary Mouse Ocular Surface CellsStem CellsUltraviolet-C (UV-C) DamageReactive Oxygen Species (ROS)DCFDAPropidium IodideHoechstLive Cell PermeantDead Cell PermeantUV CrosslinkerDose-response

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Bose, B., Kapoor, S., Sen, U., Nihad AS, M., Chaudhury, D., Shenoy P, S. Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage. J. Vis. Exp. (156), e59924, doi:10.3791/59924 (2020).

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