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Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

DOI:
10.3791/56126-v

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08:55 min

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November 20, 2017

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, , , , ,
1Developmental Biology, The Roslin Institute and R(D)SVS,University of Edinburgh, 2Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital, School of Basic Medical Sciences,Guangzhou Medical University, 3Clinical Sciences and R(D)SVS,University of Edinburgh

Chapters

  • 00:05Title
  • 00:40Extraction of Primary Rat Valve Interstitial Cells (VICs)
  • 04:49Induction of Calcification of VICs and VIC Calcification Studies
  • 06:37Results: Analysis of RAT VICs Localization, Calcification, and Associated Gene Expression Changes
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the isolation, culture, and calcification of rat-derived valve interstitial cells, a highly physiological in vitro model of calcific aortic valve disease (CAVD). Exploitation of this rat model facilitates CAVD research in exploring the cell and molecular mechanisms that underlie this complex pathological process.

Tags

Keywords: Primary Rat Valve Interstitial CellsAortic Valve CalcificationVICsCell IsolationCell CultureValve Endothelial CellsVECsCollagenase DigestionCell Model

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