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Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

9,410 Views

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08:55 min

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November 20, 2017

DOI:

10.3791/56126-v

DOI:
10.3791/56126-v

•

08:55 min
November 20, 2017

•

3 Views
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1Developmental Biology, The Roslin Institute and R(D)SVS,University of Edinburgh, 2Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital, School of Basic Medical Sciences,Guangzhou Medical University, 3Clinical Sciences and R(D)SVS,University of Edinburgh

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Primary Rat Valve Interstitial CellsAortic Valve CalcificationVICsCell IsolationCell CultureValve Endothelial CellsVECsCollagenase DigestionCell Model

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Lin, C., Zhu, D., Markby, G., Corcoran, B. M., Farquharson, C., Macrae, V. E. Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification. J. Vis. Exp. (129), e56126, doi:10.3791/56126 (2017).

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