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原发性大鼠瓣膜间质细胞的分离与鉴定: 主动脉瓣钙化的新模型研究
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
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原发性大鼠瓣膜间质细胞的分离与鉴定: 主动脉瓣钙化的新模型研究

DOI:
10.3791/56126-v

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08:55 min

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November 20, 2017

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, , , , ,
1Developmental Biology, The Roslin Institute and R(D)SVS,University of Edinburgh, 2Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital, School of Basic Medical Sciences,Guangzhou Medical University, 3Clinical Sciences and R(D)SVS,University of Edinburgh

Chapters

  • 00:05Title
  • 00:40Extraction of Primary Rat Valve Interstitial Cells (VICs)
  • 04:49Induction of Calcification of VICs and VIC Calcification Studies
  • 06:37Results: Analysis of RAT VICs Localization, Calcification, and Associated Gene Expression Changes
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了大鼠源性瓣膜间质细胞的分离、培养和钙化, 这是一种高度生理学的钙化主动脉瓣病变 (CAVD) 的体外模型。这种大鼠模型的开发有助于 CAVD 研究的细胞和分子机制的基础, 这一复杂的病理过程。

Tags

Primary Rat Valve Interstitial CellsAortic Valve CalcificationVICsCell IsolationCell CultureValve Endothelial CellsVECsCollagenase DigestionCell Model

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