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Culture and Transfection of Zebrafish Primary Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Culture and Transfection of Zebrafish Primary Cells

Culture and Transfection of Zebrafish Primary Cells

English

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11,965 Views

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08:51 min

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August 17, 2018

DOI:

10.3791/57872-v

DOI:
10.3791/57872-v

•

08:51 min
August 17, 2018

•

13 Views
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1Division of Cellular and Molecular Neurobiology, Zoological Institute,Braunschweig University of Technology, 2Department of Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics,Braunschweig University of Technology

Summary

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We present an efficient and easy-to-use protocol for preparing primary cell cultures of zebrafish embryos for transfection and live cell imaging as well as a protocol to prepare primary cells from adult zebrafish brain.

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Cite this Article

Russo, G., Lehne, F., Pose Méndez, S. M., Dübel, S., Köster, R. W., Sassen, W. A. Culture and Transfection of Zebrafish Primary Cells. J. Vis. Exp. (138), e57872, doi:10.3791/57872 (2018).

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