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リン酸化部位決定のためのオリゴペプチド競合アッセイ
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Biochemistry
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JoVE Journal Biochemistry
Oligopeptide Competition Assay for Phosphorylation Site Determination
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リン酸化部位決定のためのオリゴペプチド競合アッセイ

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8,292 Views

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09:16 min

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May 18, 2017

DOI:

10.3791/55708-v

DOI:
10.3791/55708-v

•

09:16 min
May 18, 2017

•

8 Views
, , , ,
1College of Pharmacy and Research Institute of Pharmaceutical Sciences,Seoul National University, 2College of Pharmacy and Institute of Pharmaceutical Science and Technology,Hanyang University

Summary

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ペプチド競合アッセイは、種々の分子および免疫学的実験において広く使用されている。本稿では、in vitroオリゴペプチド競合キナーゼアッセイの詳細な方法と、特定のリン酸化部位を見出すのに有用な関連する検証手順について説明します。

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Cite this Article

Joo, M. S., Koo, J. H., Shin, S., Yim, H., Kim, S. G. Oligopeptide Competition Assay for Phosphorylation Site Determination. J. Vis. Exp. (123), e55708, doi:10.3791/55708 (2017).

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