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Fosforilasyon Saha Tespiti İçin Oligopeptide Rekabet Testi
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Biochemistry
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JoVE Journal Biochemistry
Oligopeptide Competition Assay for Phosphorylation Site Determination
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Fosforilasyon Saha Tespiti İçin Oligopeptide Rekabet Testi

Türkçe

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8,292 Views

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09:16 min

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May 18, 2017

DOI:

10.3791/55708-v

DOI:
10.3791/55708-v

•

09:16 min
May 18, 2017

•

8 Views
, , , ,
1College of Pharmacy and Research Institute of Pharmaceutical Sciences,Seoul National University, 2College of Pharmacy and Institute of Pharmaceutical Science and Technology,Hanyang University

Summary

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Peptid rekabet testleri, çeşitli moleküler ve immünolojik deneylerde yaygın olarak kullanılır. Bu makale , in vitro oligopeptitle rekabet eden kinaz testi için ayrıntılı bir yöntem ve ilgili fosforilasyon sitelerini bulmak için yararlı olabilen ilişkili validasyon prosedürlerini açıklamaktadır.

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Joo, M. S., Koo, J. H., Shin, S., Yim, H., Kim, S. G. Oligopeptide Competition Assay for Phosphorylation Site Determination. J. Vis. Exp. (123), e55708, doi:10.3791/55708 (2017).

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