Journal
/
Bioquímica
/
Oligopeptide Competition Assay for Phosphorylation Site Determination
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Oligopeptide Competition Assay for Phosphorylation Site Determination

Oligopeptide Competition Assay for Phosphorylation Site Determination

8,292 Views

•

09:16 min

•

May 18, 2017

DOI:

10.3791/55708-v

DOI:
10.3791/55708-v

•

09:16 min
May 18, 2017

•

8 Views
, , , ,
1College of Pharmacy and Research Institute of Pharmaceutical Sciences,Seoul National University, 2College of Pharmacy and Institute of Pharmaceutical Science and Technology,Hanyang University

Summary

Automatically generated

Peptide competition assays are widely used in a variety of molecular and immunological experiments. This paper describes a detailed method for an in vitro oligopeptide-competing kinase assay and the associated validation procedures, which may be useful to find specific phosphorylation sites.

Explore More Videos

Oligopeptide Competition AssayPhosphorylation Site DeterminationCell SignalingPost-translational ModificationsSite-directed MutagenesisPCRDpnI DigestionBacterial TransformationDNA SequencingProtein Expression And Purification

Read Article

Cite this Article

Joo, M. S., Koo, J. H., Shin, S., Yim, H., Kim, S. G. Oligopeptide Competition Assay for Phosphorylation Site Determination. J. Vis. Exp. (123), e55708, doi:10.3791/55708 (2017).

Download .ris file

Copy

Share Video

.

Copy