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Oligopeptide Competition Assay for Phosphorylation Site Determination
Kapitel
- 00:05Titel
- 01:05Site-directed Mutagenesis of pGEX-Nrf2
- 03:45In Vitro Competitive 5′ Adenosine Monophosphate-activated Protein Kinase (AMPK) Assay
- 06:13In Vitro AMPK Activity Assay with a Site-specific Mutant
- 07:39Results: In Vitro AMPK Assays with Competitive Oligopeptides or a Site-specific Mutant Protein
- 08:31Conclusion
Peptide competition assays are widely used in a variety of molecular and immunological experiments. This paper describes a detailed method for an in vitro oligopeptide-competing kinase assay and the associated validation procedures, which may be useful to find specific phosphorylation sites.
