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Oligopeptide Competition Assay for Phosphorylation Site Determination
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JoVE Journal Bioquímica
Oligopeptide Competition Assay for Phosphorylation Site Determination

Oligopeptide Competition Assay for Phosphorylation Site Determination

8,292 Views

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09:16 min

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May 18, 2017

DOI:

10.3791/55708-v

DOI:
10.3791/55708-v

•

09:16 min
May 18, 2017

•

8 Views
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1College of Pharmacy and Research Institute of Pharmaceutical Sciences,Seoul National University, 2College of Pharmacy and Institute of Pharmaceutical Science and Technology,Hanyang University

Summary

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Peptide competition assays are widely used in a variety of molecular and immunological experiments. This paper describes a detailed method for an in vitro oligopeptide-competing kinase assay and the associated validation procedures, which may be useful to find specific phosphorylation sites.

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Joo, M. S., Koo, J. H., Shin, S., Yim, H., Kim, S. G. Oligopeptide Competition Assay for Phosphorylation Site Determination. J. Vis. Exp. (123), e55708, doi:10.3791/55708 (2017).

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