Journal
/
Biochemistry
/
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

DOI:
10.3791/55932-v

•

12:55 min

•

October 10, 2017

•

, ,
1Systems Biotechnology Research Center,KIST Gangneung Institute of Natural Products, 2Division of Bio-Medical Science & Technology, KIST School,Korea University of Science and Technology

Chapters

  • 00:05Title
  • 01:01Cell Culture, Treatment, and Examination of Protein Expression
  • 02:53Phosphate Assay
  • 05:22Microtubule-binding Assay
  • 07:55Localization and Expression of Tau After Treatment of Cells with Curcumin
  • 10:23Results: Phosphorylation, Microtubule Binding, and Localization of Tau Protein in Colorectal Cancer Cells
  • 12:03Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript describes standard protocols for measuring tau hyperphosphorylation, measuring tau binding to microtubules, and localization of intracellular tau following drug treatments. These protocols can be used repetitively for screening drugs or other compounds that target tau hyperphosphorylation or microtubule binding.

Tags

Tau PhosphorylationMicrotubule BindingTau LocalizationConfocal MicroscopyColorectal Cancer CellsPhosphatase AssayMicrotubule-binding AssayTau ProteinHCT 116 CellsCurcuminLithium ChlorideRIPA BufferBradford AssayAlkaline Phosphatase

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Oligopeptide Competition Assay for Phosphorylation Site Determination

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson’s Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Read Article