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寡肽竞争测定磷酸化位点测定

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Oligopeptide Competition Assay for Phosphorylation Site Determination

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寡肽竞争测定磷酸化位点测定

8,286 Views

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09:16 min

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May 18, 2017

DOI:

10.3791/55708-v

DOI:

10.3791/55708-v

•

09:16 min

May 18, 2017

•

8 Views

Min Sung Joo¹, Ja Hyun Koo¹, Sol-Bi Shin², Hyungshin Yim², Sang Geon Kim¹

¹College of Pharmacy and Research Institute of Pharmaceutical Sciences,Seoul National University, ²College of Pharmacy and Institute of Pharmaceutical Science and Technology,Hanyang University

Summary

肽竞争测定广泛用于各种分子和免疫学实验。本文描述了体外寡肽竞争激酶测定的详细方法和相关的验证程序，其可用于发现特定的磷酸化位点。

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