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Ensayos de Retardo de Gel de ARNt de Proteína-ARNt para el Análisis del<emN</em<sup6</sup> -threonylcarbamoyladenosine TcdA Function
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Biochemistry
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JoVE Journal Biochemistry
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
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Ensayos de Retardo de Gel de ARNt de Proteína-ARNt para el Análisis del<emN</em<sup6</sup> -threonylcarbamoyladenosine TcdA Function

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9,572 Views

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08:03 min

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June 21, 2017

DOI:

10.3791/55638-v

DOI:
10.3791/55638-v

•

08:03 min
June 21, 2017

•

10 Views
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1Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC),Spanish National Research Council (CSIC), 2Department of Immunology,Complutense University School of Medicine, 3Abvance Biotech srl

Summary

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Se presenta un protocolo para la producción de tRNA (UUU) y el análisis de tRNA (UUU) en complejo con la enzima TcdA mediante ensayos de retardo de gel de agarosa.

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Fernández, F. J., Gómez, S., Navas-Yuste, S., López-Estepa, M., Vega, M. C. Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function. J. Vis. Exp. (124), e55638, doi:10.3791/55638 (2017).

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