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Analisi del rallentamento del gel agarosio proteina-tRNA per l'analisi del<em> N</em<sup> 6</sup> -reonilcarbamoyladenosine TcdA Funzione
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Biochemistry
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JoVE Journal Biochemistry
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
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Analisi del rallentamento del gel agarosio proteina-tRNA per l'analisi del<em> N</em<sup> 6</sup> -reonilcarbamoyladenosine TcdA Funzione

italiano

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9,572 Views

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08:03 min

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June 21, 2017

DOI:

10.3791/55638-v

DOI:
10.3791/55638-v

•

08:03 min
June 21, 2017

•

10 Views
, , , ,
1Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC),Spanish National Research Council (CSIC), 2Department of Immunology,Complutense University School of Medicine, 3Abvance Biotech srl

Summary

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Viene presentato un protocollo per la produzione di tRNA (UUU) e l'analisi di tRNA (UUU) in complesso con l'enzima TcdA mediante saggi di retardazione del gel agarosio.

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Fernández, F. J., Gómez, S., Navas-Yuste, S., López-Estepa, M., Vega, M. C. Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function. J. Vis. Exp. (124), e55638, doi:10.3791/55638 (2017).

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