Journal
/
Biochemistry
/
The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

DOI:
10.3791/63088-v

•

04:50 min

•

February 08, 2022

•

, , , ,
1School of Chemistry and Molecular Biosciences,The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation,The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy,Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy,Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering,Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture,Yangzhou University, 7Glycation and Diabetes Group,Mater Research Institute-The University of Queensland, Translational Research Institute

Chapters

  • 00:00Introduction
  • 00:30Glycogen Extraction
  • 02:44Results: The Effect of Sucrose Concentration on the Particle Size of Glycogen
  • 04:25Conclusion

Summary

Automatic Translation

Automatic Translation

An optimal sucrose concentration was determined for the extraction of liver glycogen using sucrose density gradient centrifugation. The addition of a 10 min boiling step to inhibit glycogen-degrading enzymes proved beneficial.

Tags

Liver GlycogenGlycogen ExtractionGlycogen StructureDiabetesGlycogen Storage DiseaseGlycogen Isolation BufferUltracentrifugationSucrose GradientEthanol PrecipitationGlycogen Content DeterminationFreeze Drying

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Determination of the Glycogen Content in Cyanobacteria

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

Single-Molecule Tracking Microscopy – A Tool for Determining the Diffusive States of Cytosolic Molecules

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

Read Article