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Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers
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Chapters
- 00:04Title
- 00:47Preparation of the Sample
- 03:22Preparing and Running the Gel
- 04:30Transfer
- 06:07Western Blotting Detection
- 07:45Results: Use of SDD-AGE to Confirm the Presence of Heterogenous Sizes of Amyloid-like Fibers
- 09:24Conclusion
Here we use two-dimensional semi-denaturing agarose gel electrophoresis to confirm the presence of amyloid-like fibers of heterogeneous size and exclude the possibility that the size heterogeneity is due to dissociation of the amyloid fibers during the gel running process.
