Rivista
/
Biochimica
/
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
JoVE Journal
Biochimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biochimica
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function

Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function

9,561 Views

•

08:03 min

•

June 21, 2017

DOI:

10.3791/55638-v

DOI:
10.3791/55638-v

•

08:03 min
June 21, 2017

•

10 Views
, , , ,
1Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC),Spanish National Research Council (CSIC), 2Department of Immunology,Complutense University School of Medicine, 3Abvance Biotech srl

Summary

Automatically generated

A protocol is presented for the production of tRNA(UUU) and the analysis of tRNA(UUU) in complex with the enzyme TcdA by agarose gel retardation assays.

Explore More Videos

Protein-tRNA InteractionAgarose Gel Retardation AssayRNA-binding ProteinsTcdA FunctionRNA BiologyProtein-RNA ComplexesE. Coli BL21 DE3PET-23D EC Triple-UIPTG InductionCell LysisAcid Phenol Extraction

Read Article

Cite this Article

Fernández, F. J., Gómez, S., Navas-Yuste, S., López-Estepa, M., Vega, M. C. Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function. J. Vis. Exp. (124), e55638, doi:10.3791/55638 (2017).

Download .ris file

Copy

Share Video

.

Copy