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Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time
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Biochemistry
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JoVE Journal Biochemistry
Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time

2,968 Views

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07:56 min

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May 30, 2021

DOI:

10.3791/62655-v

DOI:
10.3791/62655-v

•

07:56 min
May 30, 2021

•

3 Views
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1Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Faculty of Mathematics & Science, The Edmond J. Safra Campus,The Hebrew University of Jerusalem, 2The Center for Nanoscience and Nanotechnology,The Hebrew University of Jerusalem

Tags

Time-resolvedProtein-induced Fluorescence EnhancementSingle-moleculeAlpha-synucleinStructural SubpopulationsLocal ConformationsSulfo-Cy3BSAWater Immersion ObjectiveLaser FocusPhoton DetectionFRETBurstsPhoton-HDF5

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Zaer, S., Lerner, E. Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time. J. Vis. Exp. (171), e62655, doi:10.3791/62655 (2021).

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