Journal
/
Biochemistry
/
Protein-tRNA-Agarose-Gel-Retardations-Assays für die Analyse der<em> N.</em<sup> 6</sup> -threonylcarbamoyladenosin TcdA-Funktion
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
Please note that all translations are automatically generated. Click here for the English version.

Protein-tRNA-Agarose-Gel-Retardations-Assays für die Analyse der<em> N.</em<sup> 6</sup> -threonylcarbamoyladenosin TcdA-Funktion

Deutsch

Automatically Generated

9,572 Views

•

08:03 min

•

June 21, 2017

DOI:

10.3791/55638-v

DOI:
10.3791/55638-v

•

08:03 min
June 21, 2017

•

10 Views
, , , ,
1Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC),Spanish National Research Council (CSIC), 2Department of Immunology,Complutense University School of Medicine, 3Abvance Biotech srl

Summary

Automatically generated

Ein Protokoll wird für die Produktion von tRNA (UUU) und die Analyse von tRNA (UUU) im Komplex mit dem Enzym TcdA durch Agarose-Gel-Retardierungs-Assays vorgestellt.

Explore More Videos

Protein-tRNA InteractionAgarose Gel Retardation AssayRNA-binding ProteinsTcdA FunctionRNA BiologyProtein-RNA ComplexesE. Coli BL21 DE3PET-23D EC Triple-UIPTG InductionCell LysisAcid Phenol Extraction

Read Article

Cite this Article

Fernández, F. J., Gómez, S., Navas-Yuste, S., López-Estepa, M., Vega, M. C. Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function. J. Vis. Exp. (124), e55638, doi:10.3791/55638 (2017).

Download .ris file

Copy

Share Video

.

Copy