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Developmental Biology
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分离培养成年斑马鱼脑源性神经球
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
Please note that all translations are automatically generated.
Click here for the English version.
分离培养成年斑马鱼脑源性神经球
DOI:
10.3791/53617-v
•
11:01 min
•
February 29, 2016
•
Miguel A. Lopez-Ramirez*
1,2
,
Charles-Félix Calvo*
3
,
Emma Ristori
,
Jean-Léon Thomas
3
,
Stefania Nicoli
1
Yale Cardiovascular Research Center, Internal Medicine
,
Yale University
,
2
Department of Medicine
,
University of California, San Diego
,
3
APHP Groupe Hospitalier Pitié-Salpètrière
,
Université Pierre and Marie Curie
Chapters
00:05
Title
01:02
Dissection of Adult Zebrafish Brain
03:25
Single Cell Dissociation of Adult Brain
06:07
Generation and Passaging of Primary Neurospheres
08:16
Results: Analysis of Neurospheres from Adult Zebrafish Brain
09:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提供一个可重复的方法,使用从整个脑或从任一端脑,顶盖或成年斑马鱼脑的小脑区域衍生的神经球测定以检查成人神经发生。此外,我们描述来操纵在斑马鱼神经球的基因表达的步骤。
Tags
Adult Zebrafish Brain
Neurosphere Assay
Adult Neurogenesis
Neural Stem Cell
Neural Progenitor Cell
In Vitro
Dissection
Micro-dissection
DMEM/F12 Medium
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