Journal
/
发育生物学
/
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

12,624 Views

•

11:01 min

•

February 29, 2016

DOI:

10.3791/53617-v

DOI:
10.3791/53617-v

•

11:01 min
February 29, 2016

•

8 Views
, , , ,
1Yale Cardiovascular Research Center, Internal Medicine,Yale University, 2Department of Medicine,University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière,Université Pierre and Marie Curie

Summary

Automatically generated

Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.

Explore More Videos

Adult Zebrafish BrainNeurosphere AssayAdult NeurogenesisNeural Stem CellNeural Progenitor CellIn VitroDissectionMicro-dissectionDMEM/F12 Medium

Read Article

Cite this Article

Lopez-Ramirez, M. A., Calvo, C., Ristori, E., Thomas, J., Nicoli, S. Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres. J. Vis. Exp. (108), e53617, doi:10.3791/53617 (2016).

Download .ris file

Copy

Share Video

.

Copy