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发育生物学
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Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
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发育生物学
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JoVE 杂志 发育生物学
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

DOI:
10.3791/53617-v

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11:01 min

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February 29, 2016

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, , , ,
1Yale Cardiovascular Research Center, Internal Medicine,Yale University, 2Department of Medicine,University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière,Université Pierre and Marie Curie

Chapters

  • 00:05Title
  • 01:02Dissection of Adult Zebrafish Brain
  • 03:25Single Cell Dissociation of Adult Brain
  • 06:07Generation and Passaging of Primary Neurospheres
  • 08:16Results: Analysis of Neurospheres from Adult Zebrafish Brain
  • 09:58Conclusion

Summary

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自动翻译

Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.

Tags

IsolationCultureAdult ZebrafishNeurospheresNeurogenesisGene ExpressionNeural Stem CellsSelf-renewalDeterminationTechniqueSimpleReliableIn VitroEpigenetic RegulationMouse Neurosphere ProtocolGel PacksPetri DishFilter PaperPlastic FilmMicro-dissection InstrumentsSterilized ToolsDissecting Microscope

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