Journal
/
발생학
/
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

DOI:
10.3791/53617-v

•

11:01 min

•

February 29, 2016

•

, , , ,
1Yale Cardiovascular Research Center, Internal Medicine,Yale University, 2Department of Medicine,University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière,Université Pierre and Marie Curie

Chapters

  • 00:05Title
  • 01:02Dissection of Adult Zebrafish Brain
  • 03:25Single Cell Dissociation of Adult Brain
  • 06:07Generation and Passaging of Primary Neurospheres
  • 08:16Results: Analysis of Neurospheres from Adult Zebrafish Brain
  • 09:58Conclusion

Summary

자동 번역

자동 번역

Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.

Tags

IsolationCultureAdult ZebrafishNeurospheresNeurogenesisGene ExpressionNeural Stem CellsSelf-renewalDeterminationTechniqueSimpleReliableIn VitroEpigenetic RegulationMouse Neurosphere ProtocolGel PacksPetri DishFilter PaperPlastic FilmMicro-dissection InstrumentsSterilized ToolsDissecting Microscope

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/53617?article_type=v

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury
check_url/kr/53617?article_type=v

Isolation and Characterization of Single Cells from Zebrafish Embryos
check_url/kr/53617?article_type=v

Isolation and Expansion of Adult Canine Hippocampal Neural Precursors
check_url/kr/53617?article_type=v

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
check_url/kr/53617?article_type=v

Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy
check_url/kr/53617?article_type=v

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
check_url/kr/53617?article_type=v

The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens
check_url/kr/53617?article_type=v

Culture and Transfection of Zebrafish Primary Cells
check_url/kr/53617?article_type=v

Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart
check_url/kr/53617?article_type=v

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue

Read Article