Journal
/
발생학
/
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

12,624 Views

•

11:01 min

•

February 29, 2016

DOI:

10.3791/53617-v

DOI:
10.3791/53617-v

•

11:01 min
February 29, 2016

•

8 Views
, , , ,
1Yale Cardiovascular Research Center, Internal Medicine,Yale University, 2Department of Medicine,University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière,Université Pierre and Marie Curie

Summary

Automatically generated

Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.

Explore More Videos

Adult Zebrafish BrainNeurosphere AssayAdult NeurogenesisNeural Stem CellNeural Progenitor CellIn VitroDissectionMicro-dissectionDMEM/F12 Medium

Read Article

Cite this Article

Lopez-Ramirez, M. A., Calvo, C., Ristori, E., Thomas, J., Nicoli, S. Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres. J. Vis. Exp. (108), e53617, doi:10.3791/53617 (2016).

Download .ris file

Copy

Share Video

.

Copy