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Biologie du développement
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Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
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Biologie du développement
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Journal JoVE Biologie du développement
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

12,624 Views

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11:01 min

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February 29, 2016

DOI:

10.3791/53617-v

DOI:
10.3791/53617-v

•

11:01 min
February 29, 2016

•

8 Views
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1Yale Cardiovascular Research Center, Internal Medicine,Yale University, 2Department of Medicine,University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière,Université Pierre and Marie Curie

Summary

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Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.

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Adult Zebrafish BrainNeurosphere AssayAdult NeurogenesisNeural Stem CellNeural Progenitor CellIn VitroDissectionMicro-dissectionDMEM/F12 Medium

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Cite this Article

Lopez-Ramirez, M. A., Calvo, C., Ristori, E., Thomas, J., Nicoli, S. Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres. J. Vis. Exp. (108), e53617, doi:10.3791/53617 (2016).

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