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Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart
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Developmental Biology
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JoVE Journal Developmental Biology
Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart

Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart

DOI:
10.3791/58054-v

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09:12 min

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July 29, 2018

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, , , , ,
1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine,Peking University, 2Department of Biomedical Engineering, College of Engineering,Peking University

Chapters

  • 00:04Title
  • 00:37Adult Zebrafish Ventricular Resection
  • 02:42Preparation of siRNA-encapsulated Nanoparticles and Injection of siRNA-encapsulated Nanoparticles into Adult Zebrafish Heart
  • 04:29Heart Collection, Fixation, and Evaluation of the Efficiency of siRNA Delivery
  • 05:34Evaluation of Nanoparticle-mediated siRNA Gene-silencing
  • 06:38Results: Nanoparticle-mediated siRNA Gene-silencing
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

It remains a major challenge to develop conditional gene-knockout or effective gene-knockdown in adult zebrafish organs. Here we report a protocol for performing nanoparticle-mediated siRNA gene-silencing in adult zebrafish heart, thus providing a new loss-of-function method for studying adult organs in zebrafish and other model organisms.

Tags

NanoparticleSiRNAGene-silencingZebrafishHeart RegenerationVentricular ResectionPAM Nanoparticle DendrimerALDH1A2 SiRNAScrambled Negative Control SiRNACyanine DyesAnesthesiaSurgeryRecovery

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