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Developmental Biology

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JoVE Journal Developmental Biology
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
 

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

Article DOI: 10.3791/53617-v • 11:01 min • February 29th, 2016
February 29th, 2016 •
*1,2, *3, 1, 1,3, 1
1Yale Cardiovascular Research Center, Internal Medicine, Yale University, 2Department of Medicine, University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière, Université Pierre and Marie Curie
* These authors contributed equally

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Resumen
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Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.

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Isolation Culture Adult Zebrafish Neurospheres Neurogenesis Gene Expression Neural Stem Cells Self-renewal Determination Technique Simple Reliable In Vitro Epigenetic Regulation Mouse Neurosphere Protocol Gel Packs Petri Dish Filter Paper Plastic Film Micro-dissection Instruments Sterilized Tools Dissecting Microscope
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