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Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy
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Developmental Biology
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JoVE Journal Developmental Biology
Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

DOI:
10.3791/55335-v

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11:55 min

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February 24, 2017

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, , ,
1Department of Biological Sciences,University of Notre Dame, 2Department of Neurosurgery,Mayo Clinic

Chapters

  • 00:05Title
  • 01:11Isolation and Culturing of Retinal Explants
  • 03:30Multiphoton Microscopy
  • 07:52Analysis of Velocity
  • 09:34Results: Analysis of Apical and Basal Migration Velocities
  • 10:55Conclusion

Summary

Automatic Translation

Automatic Translation

Zebrafish retinal regeneration has mostly been studied using fixed retinas. However, dynamic processes such as interkinetic nuclear migration occur during the regenerative response and require live-cell imaging to investigate the underlying mechanisms. Here, we describe culture and imaging conditions to monitor Interkinetic Nuclear Migration (INM) in real-time using multiphoton microscopy.

Tags

Retinal ExplantLive-cell ImagingMultiphoton MicroscopyZebrafishInterkinetic Nuclear MigrationRetinal RegenerationMuller GliaMicroglial BehaviorPhagocytosisAgaroseFluoroDish

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