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Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
Chapters
- 00:05Title
- 01:02Preparation of Phagocytic Targets
- 04:18Live Video Microscopy
- 05:47Calibration and Control Experiments
- 06:34Analysis
- 11:44Results: Time Courses, Average pH and Ratio Histograms of Wild-type and Hvcn1-/- Neutrophil Phagosomes
- 13:08Conclusion
Phagosomal pH influences phagosome maturation, oxidant production, phagosomal killing as well as antigen presentation. Here we describe a ratiometric method for measuring time-course and endpoint pH changes in individual phagosomes in living phagocytes using fluorescence microscopy.
