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Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging
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1Temasek Life Sciences Laboratory, 2Department of Biological Science,National University of Singapore, 3Bioimaging and Biocomputing Facility,Temasek Life Sciences Laboratory, 4Histology and Light Microscopy Core,Gladstone Institutes, 5Pacific Biosciences Research Center,University of Hawai’i at Mānoa
Chapters
- 00:05Title
- 00:35Preparing the Fly for Imaging
- 01:27Stimulation of Gustatory Neurons
- 03:01Analyzing the Data
- 04:58Results: Pheromone-induced Nueral Responses in GCaMP-expressing Nuerons
- 06:09Conclusion
The forelegs and proboscis of Drosophila contain a rich repertoire of gustatory sensory neurons. Here, we present a method using calcium imaging to measure physiological responses from sensory neurons in the foreleg and proboscis of live flies upon exogenous application of a gustatory pheromone.
