Journal
/
Immunology and Infection
/
Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

English

Automatically Generated

9,839 Views

•

12:42 min

•

April 05, 2017

DOI:

10.3791/55107-v

DOI:
10.3791/55107-v

•

12:42 min
April 05, 2017

•

10 Views
, , , ,
1Centre for Molecular Medicine, Division of Medicine,University College London, 2Cell and Developmental Biology,University College London

Summary

Automatically generated

This manuscript describes a simple method to measure the phagosomal pH and area as well as the cytoplasmic pH of human and mouse neutrophils using the ratiometric indicator seminaphthorhodafluor (SNARF)-1, or S-1. This is achieved using live-cell confocal fluorescence microscopy and image analysis.

Explore More Videos

NeutrophilPhagosomeCytoplasmPH IndicatorConfocal MicroscopyNADPH OxidasePhagocytic VacuoleIon FluxesCarboxy-S-1 Succinimidyl EsterCandidaOpsonizationHuman NeutrophilsPeripheral BloodDextran

Read Article

Cite this Article

Foote, J. R., Levine, A. P., Behe, P., Duchen, M. R., Segal, A. W. Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator. J. Vis. Exp. (122), e55107, doi:10.3791/55107 (2017).

Download .ris file

Copy

Share Video

.

Copy