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Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

9,722 Views

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12:42 min

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April 05, 2017

DOI:

10.3791/55107-v

DOI:
10.3791/55107-v

•

12:42 min
April 05, 2017

•

3 Views
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1Centre for Molecular Medicine, Division of Medicine,University College London, 2Cell and Developmental Biology,University College London

Tags

NeutrophilPhagosomeCytoplasmPH IndicatorConfocal MicroscopyNADPH OxidasePhagocytic VacuoleIon FluxesCarboxy-S-1 Succinimidyl EsterCandidaOpsonizationHuman NeutrophilsPeripheral BloodDextran

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Foote, J. R., Levine, A. P., Behe, P., Duchen, M. R., Segal, A. W. Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator. J. Vis. Exp. (122), e55107, doi:10.3791/55107 (2017).

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