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Immunology and Infection

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Study of Phagolysosome Biogenesis in Live Macrophages

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Immunology and Infection

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JoVE Journal Immunology and Infection

Study of Phagolysosome Biogenesis in Live Macrophages

Study of Phagolysosome Biogenesis in Live Macrophages

DOI:

10.3791/51201-v

•

08:06 min

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March 10, 2014

•

Marc Bronietzki, Bahram Kasmapour, Maximiliano Gabriel Gutierrez²

¹Research Group Phagosome Biology,Helmholtz Centre for Infection Research, ²Division of Mycobacterial Research,National Institute for Medical Research

Chapters

00:05Title
01:17Dextran Preloading
04:11Imaging and Time-lapse Movie Analysis
06:28Results: Lysosome to Phagosome Delivery Over Time
07:44Conclusion

Tags

Keywords: Phagolysosome Biogenesis Phagocytic Cells Phagosome Maturation Lysosome Fusion Live Cell Imaging Macrophages Phagocytosis IgG-coated Microbeads Fluorophore-conjugated Dextran Confocal Microscopy

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