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비례 형광 현미경으로 Phagosome 산도 측정
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Immunology and Infection
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JoVE Journal Immunology and Infection
Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
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비례 형광 현미경으로 Phagosome 산도 측정

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13,784 Views

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14:39 min

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December 07, 2015

DOI:

10.3791/53402-v

DOI:
10.3791/53402-v

•

14:39 min
December 07, 2015

•

9 Views
, ,
1Department of Cellular Physiology and Metabolism,University of Geneva

Summary

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Phagosomal pH influences phagosome maturation, oxidant production, phagosomal killing as well as antigen presentation. Here we describe a ratiometric method for measuring time-course and endpoint pH changes in individual phagosomes in living phagocytes using fluorescence microscopy.

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Cite this Article

Nunes, P., Guido, D., Demaurex, N. Measuring Phagosome pH by Ratiometric Fluorescence Microscopy. J. Vis. Exp. (106), e53402, doi:10.3791/53402 (2015).

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