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Immunology and Infection
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Ratiometric Imaging of Extracellular pH in Dental Biofilms
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Immunology and Infection
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JoVE Journal Immunology and Infection
Ratiometric Imaging of Extracellular pH in Dental Biofilms

Ratiometric Imaging of Extracellular pH in Dental Biofilms

DOI:
10.3791/53622-v

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13:05 min

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March 09, 2016

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1Department of Dentistry,Aarhus University

Chapters

  • 00:05Title
  • 00:51Confocal Microscopic Calibration of the Ratiometric Dye
  • 03:31Collection of In Situ-grown Dental Biofilm Samples
  • 05:12Biofilm pH Imaging
  • 06:59Digital Image Analysis
  • 10:44Results: Ratiometric Measuring of Extracellular pH in Dental Biofilms
  • 12:16Conclusion

Summary

Automatic Translation

Automatic Translation

A pH-sensitive ratiometric dye is used in combination with confocal laser scanning microscopy and digital image analysis to monitor extracellular pH in dental biofilms in real-time.

Tags

Ratiometric ImagingExtracellular PHDental BiofilmsConfocal MicroscopyC-SNARF-4HEPES BufferPH CalibrationFluorescence Imaging3D VisualizationSpatial ResolutionReal-time MonitoringBiofilm Research

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