Journal
/
免疫与感染
/
Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

DOI:
10.3791/53402-v

•

14:39 min

•

December 07, 2015

•

, ,
1Department of Cellular Physiology and Metabolism,University of Geneva

Chapters

  • 00:05Title
  • 01:02Preparation of Phagocytic Targets
  • 04:18Live Video Microscopy
  • 05:47Calibration and Control Experiments
  • 06:34Analysis
  • 11:44Results: Time Courses, Average pH and Ratio Histograms of Wild-type and Hvcn1-/- Neutrophil Phagosomes
  • 13:08Conclusion

Summary

自动翻译

自动翻译

Phagosomal pH influences phagosome maturation, oxidant production, phagosomal killing as well as antigen presentation. Here we describe a ratiometric method for measuring time-course and endpoint pH changes in individual phagosomes in living phagocytes using fluorescence microscopy.

Tags

PhagosomePHRatiometric Fluorescence MicroscopyPhagocytosisNeutrophilsFITCZymosanPH MeasurementLive-cell ImagingIntracellular PathogensPhagosomal AcidificationFluorescent Probes

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy

Study of Phagolysosome Biogenesis in Live Macrophages

Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging

Ratiometric Imaging of Extracellular pH in Dental Biofilms

“Phagosome Closure Assay” to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry

In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

Read Article