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Immunology and Infection
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Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

English

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9,199 Views

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08:55 min

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December 29, 2017

DOI:

10.3791/56745-v

DOI:
10.3791/56745-v

•

08:55 min
December 29, 2017

•

7 Views
, , ,
1Department of Tumor Immunology,Radboud University Medical Center

Summary

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This protocol describes a new method allowing for the quantitative visualization of complex formation of SNARE proteins, based on Förster resonance energy transfer, and fluorescence lifetime imaging microscopy.

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Cite this Article

Verboogen, D. R. J., Baranov, M. V., ter Beest, M., van den Bogaart, G. Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy. J. Vis. Exp. (130), e56745, doi:10.3791/56745 (2017).

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