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Comparativo In vivo Estudio de la gp96 adyuvante de la rana Xenopus laevis</em
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Chapters
- 00:00Title
- 01:24Introduction
- 02:12Elicitation and Harvest of Peritoneal Leukocytes (PLs)
- 03:43Pulsing and Adoptive Transfer of LG-6 PLs into Naive LG-6 Recipients
- 05:20Skin Graft Assay
- 07:14Tumor Transplantation Assay
- 07:56Collecting Blood Leukocytes
- 08:58Skin Graft and Tumor Transplantation Results
- 10:34Conclusion
La rana<em> Xenopus laevis</em> Ofrece una atractiva alternativa de animales no mamíferos modelo para explorar la capacidad de la proteína de choque térmico, tales como la gp96, para promover el antígeno específico de células T CD8. Se presentan métodos para el estudio<em> In vivo</em> Facilitación de presentación cruzada de antígenos de la piel y los tumores de gp96.
Tags
Gp96 AdjuvanticityComparative In Vivo StudyFrog Xenopus LaevisHeat Shock ProteinsChaperoned AntigensInnate And Adaptive T Cell ResponsesXenopus Skin Graft RejectionMHC Class Ia Restricted T Cell ResponsesTumors Down-regulating Class Ia MoleculesAdoptive Cell Transfer AssayPeritoneal LeukocytesAntigen Presenting Cells (APCs)CD8 T Cell ResponsesMinor Histocompatibility Skin AntigensXenopus Thymic Tumor 15/0MethodologyElicitationPulsingAdoptive TransferSkin Graft Transplantation AssayTumor Transplantation AssayHarvesting Peripheral Blood LeukocytesFlow CytometryProliferation Assays
