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Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

DOI:
10.3791/56514-v

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10:33 min

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October 25, 2017

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, , ,
1Institute for Medical Microbiology, Immunology and Hygiene,University of Cologne, 2Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD),University of Cologne

Chapters

  • 00:05Title
  • 01:03Salmonella typhimurium Culture
  • 02:17Coating of Bacteria with NHS-biotin/Streptavidin-conjugated Magnetic Beads
  • 04:38Bone Marrow-derived Macrophages (BMDM) Infection
  • 06:18Isolation of S. typhimurium-containing Phagosomes
  • 08:21Results: Successful Biotinylation of Bacterial and Isolation of Bacterial-containing Phagosomes
  • 09:41Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here a simple and quick method for the isolation of Salmonella typhimurium-containing phagosomes from macrophages by coating the bacteria with biotin and streptavidin.

Tags

Salmonella TyphimuriumPhagosomesMacrophagesIsolationImmunologyAntigen ProcessingIntracellular PathogensBiotinStreptavidin-conjugated Magnetic Beads

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