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Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
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Immunology and Infection
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JoVE Journal Immunology and Infection
Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

English

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9,999 Views

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05:31 min

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August 07, 2017

DOI:

10.3791/55800-v

DOI:
10.3791/55800-v

•

05:31 min
August 07, 2017

•

18 Views
, , , , , ,
1Copenhagen Prospective Studies on Asthma in Childhood (COPSAC), Herlev and Gentofte Hospital,University of Copenhagen, 2Department of Biotechnology and Biomedicine,Technical University of Denmark

Summary

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This protocol describes a noninvasive technique for the sampling of undisturbed mucosal lining fluid from the upper airways. It can be used to perform the quantification of in vivo levels of protein mediators, such as cytokines and chemokines, in subjects of all ages.

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Cite this Article

Wolsk, H. M., Chawes, B. L., Thorsen, J., Stokholm, J., Bønnelykke, K., Brix, S., Bisgaard, H. Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels. J. Vis. Exp. (126), e55800, doi:10.3791/55800 (2017).

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