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Comparative in vivo Study of gp96 Adjuvanticity in the Frog Xenopus laevis
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September 16, 2010
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The frog Xenopus laevis provides an attractive alternative non-mammalian model for exploring the ability of heat shock protein such as gp96 to promote antigen-specific CD8 T cell responses. We present methods to study in vivo facilitation of cross-presentation of skin and tumor antigens by gp96.
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Gp96 AdjuvanticityComparative In Vivo StudyFrog Xenopus LaevisHeat Shock ProteinsChaperoned AntigensInnate And Adaptive T Cell ResponsesXenopus Skin Graft RejectionMHC Class Ia Restricted T Cell ResponsesTumors Down-regulating Class Ia MoleculesAdoptive Cell Transfer AssayPeritoneal LeukocytesAntigen Presenting Cells (APCs)CD8 T Cell ResponsesMinor Histocompatibility Skin AntigensXenopus Thymic Tumor 15/0MethodologyElicitationPulsingAdoptive TransferSkin Graft Transplantation AssayTumor Transplantation AssayHarvesting Peripheral Blood LeukocytesFlow CytometryProliferation Assays
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Nedelkovska, H., Cruz-Luna, T., McPherson, P., Robert, J. Comparative in vivo Study of gp96 Adjuvanticity in the Frog Xenopus laevis. J. Vis. Exp. (43), e2026, doi:10.3791/2026 (2010).
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