/
/
Comparative in vivo Study of gp96 Adjuvanticity in the Frog Xenopus laevis
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Comparative in vivo Study of gp96 Adjuvanticity in the Frog Xenopus laevis
10,432 Views
•
11:20 min
•
September 16, 2010
DOI:
Summary
Automatically generated
The frog Xenopus laevis provides an attractive alternative non-mammalian model for exploring the ability of heat shock protein such as gp96 to promote antigen-specific CD8 T cell responses. We present methods to study in vivo facilitation of cross-presentation of skin and tumor antigens by gp96.
Explore More Videos
Gp96 AdjuvanticityComparative In Vivo StudyFrog Xenopus LaevisHeat Shock ProteinsChaperoned AntigensInnate And Adaptive T Cell ResponsesXenopus Skin Graft RejectionMHC Class Ia Restricted T Cell ResponsesTumors Down-regulating Class Ia MoleculesAdoptive Cell Transfer AssayPeritoneal LeukocytesAntigen Presenting Cells (APCs)CD8 T Cell ResponsesMinor Histocompatibility Skin AntigensXenopus Thymic Tumor 15/0MethodologyElicitationPulsingAdoptive TransferSkin Graft Transplantation AssayTumor Transplantation AssayHarvesting Peripheral Blood LeukocytesFlow CytometryProliferation Assays
Read Article
Cite this Article
Nedelkovska, H., Cruz-Luna, T., McPherson, P., Robert, J. Comparative in vivo Study of gp96 Adjuvanticity in the Frog Xenopus laevis. J. Vis. Exp. (43), e2026, doi:10.3791/2026 (2010).
Copy