A subscription to JoVE is required to view this content. Sign in or start your free trial.
12,641 Views
•
07:42 min
•
December 05, 2010
DOI:
Summary
Automatically generated
Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.
Explore More Videos
Non-invasive ImagingLeukocyte HomingLeukocyte MigrationIn Vivo ImagingTwo-photon MicroscopyHigh Resolution ImagingCell BehaviorLive MiceLeukocyte RecruitmentHost DefenseInfectionInflammatory DiseasesAutoimmune DiseasesIntravital ImagingSurgical PreparationBlood VesselsInflammationNon-invasive Single-cell ImagingLeukocyte TraffickingMouse Footpad And Phalanges
Read Article
Cite this Article
Wang, B., Zinselmeyer, B. H., McDole, J. R., Gieselman, P. A., Miller, M. J. Non-invasive Imaging of Leukocyte Homing and Migration in vivo. J. Vis. Exp. (46), e2062, doi:10.3791/2062 (2010).
Copy