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Comparative in vivo Study of gp96 Adjuvanticity in the Frog Xenopus laevis
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Chapters
- 00:00Title
- 01:24Introduction
- 02:12Elicitation and Harvest of Peritoneal Leukocytes (PLs)
- 03:43Pulsing and Adoptive Transfer of LG-6 PLs into Naive LG-6 Recipients
- 05:20Skin Graft Assay
- 07:14Tumor Transplantation Assay
- 07:56Collecting Blood Leukocytes
- 08:58Skin Graft and Tumor Transplantation Results
- 10:34Conclusion
The frog Xenopus laevis provides an attractive alternative non-mammalian model for exploring the ability of heat shock protein such as gp96 to promote antigen-specific CD8 T cell responses. We present methods to study in vivo facilitation of cross-presentation of skin and tumor antigens by gp96.
Tags
Gp96 AdjuvanticityComparative In Vivo StudyFrog Xenopus LaevisHeat Shock ProteinsChaperoned AntigensInnate And Adaptive T Cell ResponsesXenopus Skin Graft RejectionMHC Class Ia Restricted T Cell ResponsesTumors Down-regulating Class Ia MoleculesAdoptive Cell Transfer AssayPeritoneal LeukocytesAntigen Presenting Cells (APCs)CD8 T Cell ResponsesMinor Histocompatibility Skin AntigensXenopus Thymic Tumor 15/0MethodologyElicitationPulsingAdoptive TransferSkin Graft Transplantation AssayTumor Transplantation AssayHarvesting Peripheral Blood LeukocytesFlow CytometryProliferation Assays
